Team: Sanin Haverić, PhD, Head of Laboratory; Anja
Haverić, PhD; Maida Hadžić, MA;
External associate:
Professor Borivoj Galić
PhD student: Jelena Krivokapić
Activities of the Laboratory for Cytogenetics and Genotoxicology
are being realized through the scientific research and expert activities.
Research is based on two general aspects:
I cytogentetic and genotoxicological analysis of bioactive potential of
certain physical, chemical and biological agents, and
II cytogenetic and genotoxicological monitoring of human
populations in Bosnia and Herzegovina.
Expert activity of the Laboratory for Cytogenetics and
Genotoxicology aims on the prenatal and postnatal cytogenetic analysis
including chromosome analysis and karyotyping.
Both of stated activities are based on modern and actual methods
and tests of classic and applied cytogenetics. The most frequently used
tests are: chromosome aberrations analysis, cytokinesis-block micronucleus
cytome assay and sister chromatide exchange assay. As the majority of
research includes cell cultivation, this methodology is continuously applied
and optimized for various cell types. Also various methods of conventional,
differential and fluorescent staining of cells and chromosomes are being
constantly improved. Evaluation of cytotoxic and cytostatic potential of
tested agents includes application of colorimetric method in different cell
lines and cultures.
Capacities of the Laboratory for Cytogenetics and Genotoxicology
are significantly used for academic improvement and realization of numerous
graduate’s, master’s and doctorate thesis of graduate, postgraduate and PhD
students of University of Sarajevo.
Laboratory for
cytogenetics and genotoxicology provides following expert activities:
-
Karyotyping
–
chromosome banding and analysis;
-
Cytogenetic
biodosimetry -
chromosome aberration
analysis in peripheral blood samples of humans exposed to ionizing
radiation.
-
Genotoxicity and cytotoxicity tests in vitro (testing of genotoxic and cytotoxic potential of chemical substances, herbal extracts)
-
Chromosome
aberrations analysis;
-
Cytokinesis-block micronucleus
cytome assay;
-
Sister-chromatid
exchange assay;
-
Allium assay;
-
Alamar blue assay;
- Trypan blue assay.
-
Chromosome
aberrations analysis;
-
Establishment of primary cell lines.
Projects
·
Analysis of K2(B3O3F4OH)
bioactive and medical potential; (ongoing project).
·
Analysis of natural bioactive compounds potential in the inhibition
of genotoxic and cytotoxic effects in vitro; (2012-2013) financed by Federal ministry of education and
science.
·
Cytotoxicity and genotoxicity analysis of natural and synthetic
food colorants in FB&H; (2011-2012) financed by Federal ministry of
education and science.
·
Evaluation of antitumor properties of halogenated boroxine;
(2010-2011) financed by Federal ministry of education and science.
·
Participation in international collaborative project: HUMNXL
– Exfoliated cells micronucleus project; (2009-2011);
·
Analysis of the specific chromosomal markers of basal cell
carcinoma; (2007-2009) financed by the Ministry of Education and Science of
Sarajevo Canton;
·
Cytogenetic markers in human populations of FB&H as possible
bioindicators for Balcan syndrome; (2002-2003) financed by Federal ministry
of science, culture and sport.
Contact: lcg@ingeb.unsa.ba;
sanin.haveric@ingeb.unsa.ba;
anja.haveric@ingeb.unsa.ba;
maida.hadzic@ingeb.unsa.ba.