Expert activities



LABORATORY FOR BIOINFORMATICS AND BIOSTATISTICS

Laboratory of Bioinformatics and Biostatistics offers services in the use of bioinformatics analysis and biostatistics. Center for bioinformatics (CEBIO) that works within the Laboratory has modern computer equipment necessary to provide services in field of bioinformatics and biostatistics.

Contact: cebio@ingeb.ba.

CYTOGENETICS AND GENOTOXICOLOGY

Laboratory for Cytogenetics and Genotoxicology provides following expert activities: 

- Karyotyping (chromosome aberration analysis and karyogram making) from 

  • samples of peripheral blood; 
  • bone marrow; 
  • prenatal tissues (amniotic fluid, fetal placenta, umbilical cord blood); 
- Cytogenetic biodosimetry (chromosome aberration analysis and micronucleus assay from human peripheral blood samples);

- Genotoxicity test in vitro
  • standard chromosome aberration analysis; 
  • micronucleus cytokinesis-block test; 
  • sister-chromatid exchange test; 
  • Comet assay; 
  • neutral red test; 
  • Allium test; 
  • Alamar blue test. 
- Establishment of primary cell lines.

Contact: lcg@ingeb.ba

FORENSIC GENETICS

On global scale, the DNA forensic expertise have been established for many years as standard methods with a wide range of application in various investigative procedures, whether it is a kinship test, paternity testing or analysis of contentious biological traces collected from a crime scene. In the modern investigative processes, DNA analysis is an unavoidable method which provides highly reliable results as well as undoubtful practical values. 

Forensic genetics laboratory activities particularly include:

  • testing of disputed paternity using samples of buccal swab (which reduces traumatic effect on children), blood, hair, bones and other baseline samples.

  • motherless paternity testing,

  • testing of motherhood without the presence of the father,

  • biological kinship testing,

  • forensic DNA analysis for police, prosecution, law offices, courts and private requests. The analysis of samples of different origin is possible: skeletal remains, blood stains (on different materials), hair, semen, controversial traces on cigarette butts, controversial traces under fingernails, in urin etc.

All the above mentioned analysis is applied on 15 loci, of which 13 match with the CODIS loci, which allow result comparison with DNA databases of all world laboratories.

Contact: lfg@ingeb.ba

HUMAN GENETICS

In the Laboratory for Human Genetics (LHG) it is possible to perform genotyping – genetic characterization of a wide spectrum of diseases and disorders. This is extremely important for the correct medical diagnosis, confirmation or exclusion of suspected genetically caused condition, early detection of disease, successful therapy or pharmacological treatment of many diseases. Contemporary methods of molecular genetics are also used worldwide in early evaluation of the effect of treatment or transplantations and they can even enable prediction of response to specific therapy 

Laboratory performs verified analytical methods such as:

  • prenatal DNA characterization;

  • early determination of fetal sex and fatherhood;

  • PCR genotyping of HLA system;

  • detection of markers for different subtypes of acute myeloid (AML), acute lymphoblastic (ALL) and chronic myeloid leukemia (CML);

  • neuromuscular dystrophies (Duchenne and Becker type);

  • HPV genotyping of some of the most frequent low-risk (HPV 11) and high-risk (HPV 16, 18, 31, 33 and 35) HPV types;
  • genotyping of the entire spectrum of inherited disorders through cooperation with laboratories specialized for the detection of rare hereditary disorders or diseases (www.genetests.org).

Contact: lhg@ingeb.ba

GMO AND FOOD BIOSAFETY 

Laboratorija for GMO and food biosafety provides following expert activities :

  • Qualitative and quantitative analysis of foodstuff to the presence of DNA of specific origin;

  • DNA identification of bacterial types included in processes of production and degradation of foodstuff.

Laboratory for GMO and Food Biosafety has capacities for performing many analyses, such as:

  • Detection on the presence of pork origin DNA in food products;

  • Detection on the presence of animal origin DNA (beef, mutton, kid, bird) in feed;

  • DNA identification of bacterial types;

  • Qualitative and quantitative analysis of the presence of GMOs in food products at the DNA and protein level;

  • Identification of GMOs present in food products.


Contact: lgb@ingeb.ba

MOLECULAR GENETICS

Laboratory for Molecular Genetics of Natural Resources offers expertise in:

  • Genetic identification of the fish stock and fry of salmonid species (for stocking or restoration of the fish stock);

  • Selective fish farming (salmonid species) based on molecular genetic markers;

  • Genetic characterization of indigenous varieties of B&H fruits and vegetables using molecular genetic markers;

  • Genetic characterization of B&H autochthonous breeds of cattle using molecular genetic markers;

  • Genetic characterization of B&H native species of forest trees using molecular genetic markers.

Laboratory for Molecular Genetics of Natural Resources of the Institute for Genetic Engineering and Biotechnology provides expert services in the genetics of stocking and breeding of freshwater fish. Our services include the following analyses:

  • test of autochthony of fish intended for restocking, and quality testing of brood stock (brown trout, softmouth trout, marble trout, Danube salmon and grayling));
  • genetic test of origin (paternity testing) of the fry in case of selling or purchasing fish fry intended for commercial farming (brown trout, softmouth trout, rainbow trout, brook trout, Arctic charr, marble trout, Danube salmon and grayling));
  • selective breeding - selection of the brood stock males and females fittest for mating according to their genetic parameters, especially their indigenous origin and growth rate (brown trout, softmouth trout, marble trout, Danube salmon, grayling and carp);
  • hybridization test for all salmonoids and common cyprinids, in order to remove potential hybrids from brood stock or to confirm the success of hybridization.

All the above analyses are performed in accordance with EU regulations and standards. Professionally verified, written report is issued upon the analyses

Contact: lmg@ingeb.ba